Nadia Ameen, MBBS

  • Associate Professor of Pediatrics (Gastroenterology)
Research interests
Intestinal Diseases
Research summary

My laboratory has been supported continuously by NIH awards (K08, R03, R01) to study intracellular trafficking routes and mechanisms and that regulate the expression and function of the cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel in the intestine. CFTR represents the primary exit pathway responsible for anion and fluid secretion on the apical membranes of intestinal cells. Mutations in the CFTR gene result in absence of functional CFTR channels and the genetic disease Cystic Fibrosis while up-regulation of CFTR function is implicated in the pathogenesis of diarrheal disease. We employ transgenic animal and polarized intestinal cell models in conjunction with cell biologic, molecular and physiologic approaches to understand the intracellular trafficking routes traversed by CFTR and how alterations in these pathways lead to intestinal diseases.
Education
  • MBBS, University of West Indies, Jamaica, 1985
Publications
  • Functional vacuolar ATPase (V-ATPase) proton pumps traffic to the enterocyte brush border membrane and require CFTR. Collaco AM, Geibel P, Lee BS, Geibel JP, Ameen NA. Am J Physiol Cell Physiol. 2013 Nov 1;305(9):C981-96. doi: 10.1152/ajpcell.00067.2013. Epub 2013 Aug 28.
  • Characterization of CFTR High Expresser cells in the intestine. Jakab RL, Collaco AM, Ameen NA. Am J Physiol Gastrointest Liver Physiol. 2013 Sep 15;305(6):G453-65. doi: 10.1152/ajpgi.00094.2013. Epub 2013 Jul 18.
  • Md. Kaimul Ahsan, Boris Tchernychev, Marco M. Kessler, Robert M. Solinga, David Arthur, Cristina I. Linde. Inmaculada Silos-Santiago, Gerhard Hannig and Nadia A. Ameen. Linaclotide activates guanylate cyclase-C/cGMP/protein kinase-II dependent trafficking of CFTR in the intestine Physiological Reports, 5 (11), 2017.
  • Vandana Kumari, Shruti Desai and Nadia A. Ameen. AP2 α modulates cystic fibrosis transmembrane conductance regulator function in the human intestine. Journal of Cystic Fibrosis, 16: 327-334, 2017.
  • Dmitri V. Kravtsov, Md Kaimul Ahsan, Vandana Kumari, Sven C.D. van Ijzendoorn, Miguel Reyes-Mugica, Anoop Kumar, Tarunmeet Gujral, Pradeep K. Dudeja and Nadia A. Ameen. Identification of ion transport defects in Microvillus Inclusion Disease. Am. J Physiol, Jul 1, 311 (1) 2016.
  • Dmitri Kravtsov, Anastasia Mashukova, Radia Forteza, Maria M. Rodriguez, Nadia A. Ameen1 *, Pedro J. Salas. Myosin 5b loss of function leads to defects in polarized signaling: Implication for Microvillus Inclusion Disease pathogenesis and treatment. Am J Physiol Gastrointest Liver Physiol, 307 (10): G992-G1001, 2014.
  • Anne Collaco, Peter Geibel, Beth Lee John Geibel and Nadia Ameen. Functional Vacuolar ATPase (V-ATPase) proton pumps traffic to the enterocyte brush border membrane and require CFTR. Am J Physiol Gastrointest Liver Physiol, 305:C 981-996, 2013.
  • Peter S. Hegan, Dmitri Kravtsov, Christina Caputo, Marie E. Egan, Nadia A. Ameen and Mark S. Mooseker. Restoration of cytoskeletal and membrane tethering defects but not defects in membrane trafficking in the intestinal brush border of mice lacking both myosin Ia and myosin VI. Cytoskeleton; August 19, 2015.
  • Jakab RL, Collaco AM, Ameen NA. Characterization of CFTR High Expresser cells in the intestine. Am J Physiol Gastrointest Liver Physiol. 305: G453-465, 2013.
  • Anne Collaco, Robert Jakab, Nadia Hoekstra, Kisha Mitchell, Amos Brooks and Nadia Ameen. Regulated traffic of anion transporters in mammalian Brunner’s glands: a role for water and fluid transport. Am J Physiol Gastrointest Liver Physiol; Aug; 305(3): G258-75, 2013.