OCR3105/5254 pertains to the creation of a simple tool kit for the efficient site specific, phosphorylation signal-independent, introduction of phosphoserine into proteins in vitro and in vivo using a novel vector compatible with complementary bacterial strains and mammalian tissue culture.
Non-destructive MRI imaging of solids and MR image reconstruction algorithms.
A novel tetrodotoxin resistant sodium channel is described, along with isolated nucleotides that encode this receptor. Methods for identifying agents that modulate the Na.sup.+ current through the receptor are provided, as well as related therapeutic and diagnostic methods.
The invention provides isolated N-methyl-D-aspartate type 3B (NR3B) polypeptides, functional fragments and peptides, encoding nucleic acid molecules and polynucleotides, and specific antibodies. Also provided are excitatory glycine receptors, containing either NR3B or NR3A polypeptides. Further provided are methods for detecting excitatory glycine receptor ligands, agonists and antagonists. The invention also provides related diagnostic and therapeutic methods.
The present invention relates to previously unknown biological roles of Nogo-B. We have discovered that Nogo-B is a component of endothelial cells. We have also discovered that Nogo-B is highly expressed in intact blood vessels. The amino terminus of Nogo-B promotes the adhesion, spreading and migration of endothelial cells and plays a role in vascular remodeling. Thus, Nogo-B is a novel regulator of vascular homeostasis and remodeling. The present invention provides compositions comprising Nogo-B and fragments and fusion proteins thereof. The present invention also relates to nucleic acids encoding Nogo-B and fragments and fusion proteins thereof, as well as vectors and cells comprising such nucleic acids. The present invention also relates to antibodies specific for Nogo-B and fragments and fusion proteins thereof. The present invention also provides methods for preventing, detecting and treating Nogo-B-related diseases, disorders and conditions.
Nogo-B is a newly-discovered protein that is highly expressed in endothelial and smooth muscle cells of the vessel wall. Nogo-B has been shown to be a regulator of cell migration in vitro and of vascular remodeling in vivo. Molecules that mimic the activity of Nogo-B have been shown to be pro-angiogenic. More recently, the receptor for Nogo-B has been identified. Antagonists of the receptor have been shown to be anti-angiogenic, and therefore useful for the treatment of solid tumors. Work at Yale is underway to identify additional anti-angiogenic molecules.
The advent of fluorescent proteins (FPs) has redefined the use of fluorescence microscopy in every biological discipline. Green fluorescent protein (GFP) and its variants are commonly used in celluar imaging studies. Recently, a novel fluorescent protein, named vivid Verde Fluorescent Protein (VFP), was isolated from Cyphastrea microphthalma, a scleractinian coral found in the warmer waters of the Australian Great Barrier Reef. The VFP sequence was modified for optimal fluorescence for use in a variety of molecular and biological applications.
The invention is based on the discovery of various new IFT particle polypeptides and the genes that encode them.